Phycobilisome Degradation Protein

Protein knowledgebase. Research output: Contribution to journal › Article. During the last two years Prof. Here, we demonstrate that this photoprotective mechanism, characterized by blue light-induced fluorescence quenching, is indeed phycobilisome-related and that a soluble carotenoid binding protein, ORANGE CAROTENOID PROTEIN (OCP), encoded by the slr1963 gene in Synechocystis PCC 6803, plays an essential role in this process. consists of allophycocyanin of the protein, which is influenced by parameters such as light, (APC) cores surrounded by c-phycocyanin (CPC) peripherally. To induce state transitions in WT and mutants, we applied light of different quality: the phycobilisome-absorbed light (620 nm - light 2), and the light mainly absorbed by chlorophyll-protein complexes in PSI and PSII cores (440 and 680 nm - light 1). Induced protein degradation is an emerging drug discovery platform with the potential to reduce drug exposure requirements, counteract compensatory protein expression and target the 'undruggable. The largest differences in protein content between white and far-red light conditions reflect the changes to photosynthetic complexes, shifting from a standard phycobilisome and Chl a-based light harvesting system under white light, to modified, red-shifted phycobilisomes and Chl f-containing photosystems under far-red light conditions. Although highly fluores-. Phycobilisome linker proteins are phosphorylated in Synechocystis sp. The complementing gene, designated nblB , is expressed at approximately the same level in cells grown in nutrient-replete medium and medium devoid of either sulfur or nitrogen. Read more. PCC 7942 nitrogen regulator PipX interacts in a 2-oxoglutarate 2 dependent manner with the global nitrogen transcription factor NtcA and the signal 3 transduction protein PII. 2013 Researchers The role of a novel antisense transcript in phycobilisome degradation in Anabaena sp strain PCC 7120. lactis using the NICE system can also effectively be performed at the industrial-scale of fermentation. To record the issues published in the Journal to the institutional Repository, permission by this Society is required. 5kDa heat shock protein expressed in cyanobacterial cells, has been shown to provide functional stability to the phycobilisome during heat stress. PubMed abstract & fulltext for subscribers. The Orange Carotenoid Protein (OCP) is a photoactive water soluble protein that is crucial for photoprotection in cyanobacteria. PCC 6803 results in dramatic cellular effects. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool. The NblA protein (~6 kDa) has been identified as an essential component in this process. Involved in phycobilisome (PBS) degradation during nutrient deprivation. Phycobilisome degradation leads to a color change of the cells from blue-green to yellow-green, referred to as bleaching or chlorosis. Both have specific antenna complexes and the phycobilisome (PBS) is the major antenna protein complex in cyanobacteria, typically consisting of a core from which several rod-like subcomplexes protrude. PCC 7942 nitrogen regulator PipX interacts in a 2-oxoglutarate 2 dependent manner with the global nitrogen transcription factor NtcA and the signal 3 transduction protein PII. The defect in phycobilisome degradation was observed in the sll1961 mutant despite the increased expression of nblA, a gene involved in phycobilisome degradation during nitrogen starvation. org/network/32051. 1 N NaOH (with a final pH of 11. dnaN protein network: https://string-db. In doing so, the degradation tag effectively decreases the protein half-life or the typical length of time that a protein, once translated, will exist in the cell. We have designed a microfluidic device in which we can manipulate, lyse, label, separate, and quantify the protein contents of a single cell using single-molecule fluorescence counting. The orange carotenoid protein is one of the components that prevent on a short time scale such damage by quenching excitons before transfer to the chlorophyll proteins. The complementing gene, designated nblB , is expressed at approximately the same level in cells grown in nutrient-replete medium and medium devoid of either sulfur or nitrogen. by interactions with neighbouring pigments and the LHCII protein are crucial for. Laufman, R. The genes significantly down-regulated included the cytochrome c553 precursor (petJ) and a putative phycobilisome degradation protein (nblA). NblA, a low-molecular-weight protein, is essential for degradation of the phycobilisome. The defect in phycobilisome degradation was observed in the sll1961 mutant despite the increased expression of nblA, a gene involved in phycobilisome degradation during nitrogen starvation. 30 0 0 7,266. In: Methods in Enzymology Vol. , Katoh (1988) Phycobilisome stability. Phycobilisome degradation leads to a color change of the cells from blue-green to yellow-green, referred to as bleaching or chlorosis. consists of allophycocyanin of the protein, which is influenced by parameters such as light, (APC) cores surrounded by c-phycocyanin (CPC) peripherally. stabilization of phycobilisomes In agreement with published studies (e. the nblA gene, an essential element for phycobilisome degradation in Synechococcus 2973, as a target for dele-tion [32]. (1993) Characterization of gene clusters encoding the fucoxanthin chlorophyll protein of the diatom Phaeodactylum tricornutum. 3390/biom9080374. InterPro provides functional analysis of proteins by classifying them into families and predicting domains and important sites. , arbitrary units. Kalim Akhtar2,6,* In metabolic engineering, the production of industrially relevant chemicals, via. This subpopulation would have been missed by traditional techniques such as gel assays or mass spectrometry, which mea-sure only the mean protein concentrations. Binding assays showed interaction between Slr1098. Oxygenic photosynthesis is driven by photosystems I and II (PSI and PSII, respectively). (1994) A small peptide elicites the degradation of phycobilisomes during nutrient-limited growth of cyanobacteria. NblA in phycobilisome degradation. Therefore, the aim of this study was to test whether protein production in L. N2 - We investigated the relation between the carotenoid composition and the structure of phycobilisome (PBS) antenna of cyanobacterium Synechocystis sp. We propose that Sll1961 is a bi‐functional protein that regulates both photosystem stoichiometry under high light condition and PBS degradation under nitrogen starvation. Christoph Grundner received his PhD from the University of Heidelberg, Germany, for work on HIV vaccine design at the Dana-Farber Cancer Institute, Harvard Medical School, in Boston. The defect in phycobilisome degradation was observed in the sll1961 mutant despite the increased expression of nblA, a gene involved in phycobilisome degradation during nitrogen starvation. Generic labeling of proteins is achieved through fluorescent-antibody binding. Taken together, the results indicate that PipX interacts with NtcA to facilitate efficient acclimation of cyanobacteria to conditions of nitrogen limitation. We respectively link a Gal4 binding domain with CikA or SasA to find the suitable binding protein that can characterize the oscillation. Although highly fluores-. Figure 3C displays the expression profile of MITF proteins after Cpc treatment. protein in phycobilisome degradation. Phycobilisome linker proteins are phosphory-lated in Synechocystis sp. Role of the Synechococcus PCC 7942 nitrogen regulator protein PipX on NtcA controlled processes Javier Espinosa 1, Karl Forchhammer2, and Asunción Contreras1* 1 División de Genética, Universidad de Alicante, Apartado 99, E-03080 Alicante, Spain 2 Institut für Mikrobiologie und Molekularbiologie, Universität Giessen, Heinrich-Buff-. Insights into Functional Diversity in Neurospora This proposal investigates the genetic bases of fungal thermophily, biomass-degradation, and fungal-bacterial interactions in Sordariales, an order of biomass-degrading fungi frequently encountered in compost and encompassing one of the few groups of thermophilic fungi. In vivo, PipX is involved in the NtcA-dependent induction of 4 glnB and glnN genes. 25), in reaction with 4% (w/v) copper(II) sulfate pentahydrate 27, using bovine serum albumin as a protein. Protein homogeneity was determined by densitometric analysis. 30 Phycobilisome degradation protein (NblA) 60 7,266. affect recovery ntca-dependent induction glnn gene various nitrogen-regulated process nitrate utilization phycobilisome degradation global nitrogen transcription factor ntca decreased nitrate dependent manner signal transduction protein pii nitrate transport pipx-deficient mutant ammonium addition nitrate-containing medium pipx inactivation. These are the phycoerythrins (PE) and allophycocyanins (APC). (i) The concomitant directing of carbon excess (mainly from protein degradation) into glycogen synthesis allows the adjustment to an adequate cellular 2-OG concentration under conditions of nitrogen depletion, which in turn leads to an activation of the nitrogen signaling cascade, in particular of the PII signaling protein (Muro-Pastor et al. "The Proteolysis Adaptor, NblA, Binds to the N-Terminus of Β-Phycocyanin: Implications for the Mechanism of Phycobilisome Degradation. Protein synthesis, folding, and degradation are an accurately regulated process occurring in every organism and called proteostasis. , Deruelles, J. The spectral property of phycobiliproteins are mainly dictated by their prosthetic groups , which are linear tetrapyrroles known as phycobilins. Methods and compositions are provided for use of modified or intact phycobilisomes as extremely potent labels in sensitive monitoring kits (e. Underlying molecular mechanisms in its genesis remain unclear and optimal animal models are yet to be established. However, the mechanism of how a small 7kDa protein triggers the degradation of <7MDa protein complex is unclear. The second part of the life cycle involves the control of the expression levels of a protein by regulating its degradation. The mode of action of NblA in this degradation process is poorly understood. Previous ensemble measurements have shown that this degradation starts from the. Fast D1 degradation and synthesis were confirmed by the pulse-chase and immunoblot experiments. , Hattori, A. NblA mutants exhibit high absorbance of rod pigments under conditions that generally elicit phycobilisome degradation, implicating NblA in degradation of these pigments. FEBS Letters (582) p. Firstly mature HhoA, HhoB and HtrA from Synechocystis sp. Spectral characteristics. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5. UniProt Consortium European Bioinformatics Institute Protein Information Resource SIB Swiss Institute of Bioinformatics UniProt is an ELIXIR core data resource Main funding by: National Institutes of Health The European Molecular Biology Laboratory State Secretariat for Education, Research and Innovation SERI. Thanks to C. It is an accessory pigment to chlorophyll. NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease Article in Journal of Biological Chemistry 283(47):32394-403. protein biosynthesis and the degradation of cellular proteins, whereas the reversion to the original level during the repletion may result from the recovery of protein biosynthesis. To date the identity of the protease(s) involved in phycobilisome degradation has remained elusive. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. Phycobilisome degradation protein; (NblA) 64 7,411. Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana Douglas A. In the filamentous strain Tolypothrix sp. Cockshutt • Olga Zhaxybayeva • Hongyan Wu • Gang Li Received: 28 January 2013/Accepted: 1 March 2013 Springer Science+Business Media Dordrecht 2013 Abstract All oxygenic photoautotrophs suffer photoin-. They bind to the N-terminal domain of HtpG specifically without affecting its ATPase activity. The method of attachment may be optimized to achieve a preferred orientation of the phycobilisomes relative to the solid surface. Protein microarrays are an emerging technology for studying protein expression profiling and protein functions. In doing so, the degradation tag effectively decreases the protein half-life or the typical length of time that a protein, once translated, will exist in the cell. After an initial period dominated by phycobilisome degradation, and by a mechanism which is still not clear, UVR induced the synthesis of new phycobiliproteins and the reassembly of phycobilisomes from newly synthesized phycobiliproteins and remaining free phycobiliproteins with a peak after 30 min of exposure. Insights into Functional Diversity in Neurospora This proposal investigates the genetic bases of fungal thermophily, biomass-degradation, and fungal-bacterial interactions in Sordariales, an order of biomass-degrading fungi frequently encountered in compost and encompassing one of the few groups of thermophilic fungi. coli cells. The spatial segregation of kinases, phosphatases, proteases, and ribosomes ensures a certain order of events with minimal mutual interference. NblA most likely acts as an adaptor protein that guides a Clp protease to the phycobiliproteins, thereby initiating the degradation process. Characterization of a Transcriptional Regulatory Gene Involved in Dibenzofuran Degradation by Protein of a Novel Glucagon-Like Induced Phycobilisome. "The Proteolysis Adaptor, NblA, Binds to the N-Terminus of Β-Phycocyanin: Implications for the Mechanism of Phycobilisome Degradation. ABSTRACT 1 The Synechococcus sp. This study identifies a novel component, NblC, which mediates phycobilisome degradation under nitrogen, sulphur and phosphorus starvation. , Hernández-Prieto, M. 1093-1096 4 p. Quantitative proteomics revealed that acclimation requires, besides remodeling of the photosynthetic apparatus, also adjustment of the machinery for protein folding, degradation, and homeostasis. Since the phycobilisome is an abundant component of the cell, its degradation may provide a substantial amount of nitrogen to nitrogen-limited cells. C-phycocyanin has a single absorption peak at ~621 nm, varying slightly depending on the organism and conditions such as temperature, pH, and protein concentration in vitro. Porankiewicz & Clarke (1997) Induction of the heat shock protein ClpB affects cold acclimation in the. The Synechocystis sp. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. In unicellular non-diazotrophic cyanobacteria, NblA is a small polypeptide required for phycobilisome degradation during macronutrient limitation. NblA, a low-molecular-weight protein, is essential for degradation of the phycobilisome. The third plasmid contains CikA, SasA and RpaA. The absorbed wavelengths, energy transfer efficiency, and time before emission depend on both the fluorophore structure and its chemical environment, as the molecule in its excited state interacts with surrounding molecules. , a nblA gene (nblAI) lies upstream of the cpeBA operon that encodes phycoerythrin apoproteins. Research output: Contribution to journal › Article. degradation is a rapid, specific, and extensive process given the large amount of protein complexes involved in the process. Cloning and characterization of the Schizosaccharomyces pombe homologues of the human protein Translin and the Translin-associated protein TRAX. Nagarajan, A. Reversible inactivation of PSII reaction centers was earlier shown to occur during the initial state of photoinhibition not related to D 1 protein degradation; one of the reasons for PSII reversible inactivation is accumulation of reducing equivalents in PSII acceptor site [44, 45, 47]. However, the mechanism of how a small 7kDa protein triggers the degradation of <7MDa protein complex is unclear. Nicora1, Himadri B. These results suggest that the NblB polypeptide may be a constitutive part of the machinery that coordinates phycobilisome degradation with environmental conditions. phycobilisome degradation protein NblA: Graphical image of fluorescence kinetics : High: cells grown under 200 micro-mol photons m-2 s-1: Low: cells grown under 20. Previous ensemble studies have shown that cyanobacteria respond to changes in nutrient availability by modifying the structure of PBS complexes, but this process has not been visualized for individual pigments at the single-cell level due to spectral overlap. Phycobilisome degradation protein; (NblA) 64 7,411. It is an accessory pigment to chlorophyll. These mutants have a phenotype that can be detected visually. Cloning and characterization of the Schizosaccharomyces pombe homologues of the human protein Translin and the Translin-associated protein TRAX. and Aro, E. This phycobili-protein also induces HeLa cell apoptosis [5,6] enhances wound healing [7], retards platelet aggregation [8,9] and acts as a photodynamic agent to eradicate cancer cells. Such degradation products were also seen in Arabidopsis (Zhang et al. DsppA1 mutant strain demonstrated the loss of some adaptation mechanisms to various light intensities, that resulted in the reduced degradation of phycobilisome antenna, visualized as a less bleaching phenotype under middle light intensities (150 µE m-2 s-1), but rapidly bleaching when transferred to strong light regimes (700 µE m-2 s-1). Protein separation from the integrated microfluidic chip showing the electropherogram of protein separation of PBS from 10 cells. Additionally, the rapid degradation of NblA::GFP compared with free GFP (Figure 4) denoted that NblA was capable of designating the fusion protein to proteolysis. Each phycobilisome consists of a core made of allophycocyanin, from which several outwardly oriented rods made of stacked disks of phycocyanin and (if present) phycoerythrin(s) or phycoerythrocyanin. The NblA protein (~6 kDa) has been identified as an essential component in this process. The NblA protein (approximately 6 kDa) has been identified as an essential component in this process. 3% P i than 3% P i cultures. , 1979, supra), isolated phycobilisomes were shown to be unstable to decreases in protein concentration and ionic strength. 2008) and C-termini (Bienert et al. In particular, we found two types of genes that have never been reported in red algae: phycobilisome linker proteins and phycobilisome-degradation protein (NblA) that match to cyanobacterial homologs. The small protein NblA triggers PBS degradation in heterocysts. Accumulation of undegraded proteins in aggrosomes/inclusion bodies, or the premature degradation of (mutant) proteins is often associated with development of diseases, like cardiac and skeletal muscle myopathies. Third, in the prokaryotic cyanobacteria and eukaryotic red algae, light harvesting is carried out primarilyby agroup of. PBS is a large soluble protein complex enhances the light harvesting efficiency of the cells. Karradt et al. These results suggest that the NblB polypeptide may be a constitutive part of the machinery that coordinates phycobilisome degradation with environmental conditions. The proteolysis adaptor, NblA, binds to the N-terminus of β-phycocyanin: Implications for the mechanism of phycobilisome degradation. Some genes potentially associated with the degradation and/or transport of peptides and/or proteins were only differentially expressed within a single HMWDOM treatment. the chlorophyll-binding function of both protein families. The third plasmid contains CikA, SasA and RpaA. Schwarz, D. It has been previously shown that NblA binds via a highly. NblA, a Key Protein of Phycobilisome Degradation, Interacts with ClpC, a HSP100 Chaperone Partner of a Cyanobacterial Clp Protease. The orange carotenoid protein (OCP) is a structurally and functionally modular photoactive protein involved in cyanobacterial photoprotection. Distribution and Nomenclature of Protein-coding Table 1 summarizes the protein-coding gene content in the completely nblA CC Phycobilisome degradation prot. Nagarajan, A. Christoph Grundner received his PhD from the University of Heidelberg, Germany, for work on HIV vaccine design at the Dana-Farber Cancer Institute, Harvard Medical School, in Boston. A single type of constituent phycobilisome protein, either linker protein or phycobiliprotein, may be used as the attaching moiety to the solid support. Laufman, R. This study identifies a novel component, NblC, which mediates phycobilisome degradation under nitrogen, sulphur and phosphorus starvation. , arbitrary units. NblA is essential for phycobilisome degradation in Anabaena sp. In this study we identified a novel mutant of Synechococcus elongatus PCC 7942 that exhibits impaired phycobilisome degradation specifically during nitrogen starvation, unlike previously described mutants, which exhibit aberrant. This work provided biochemical characterization of HhoA, HtrA and HhoB from Synechocystis sp. Insights into Functional Diversity in Neurospora This proposal investigates the genetic bases of fungal thermophily, biomass-degradation, and fungal-bacterial interactions in Sordariales, an order of biomass-degrading fungi frequently encountered in compost and encompassing one of the few groups of thermophilic fungi. phycobilisome components as if the environ-ment never changed. In this study, phycobilisome-related genes, such as an R-phycoerythrin γ-subunit gene, were mainly predicted rather than chlorophyll-related genes. 4 Degradation and synthesis of new phycobilisome induced by UVR. But what is. We have not We can therefore assume that the 15 kDa band corre- observed however, any proteolytic activity associated to sponds either to a NblAI oligomer or to a complex formed NblAI in our in vitro preparations, ruling out the possibility between NblAI and another small polypeptide. This phycobili-protein also induces HeLa cell apoptosis [5,6] enhances wound healing [7], retards platelet aggregation [8,9] and acts as a photodynamic agent to eradicate cancer cells. The phycobilisome, the cyanobacterial pigment antenna, is a supramolecular assembly that may reach 4 MDa. Protein structure prediction was performed in the Zhang Server by using a template of PDB entry 1B33 without any additional restraints as guides (34 – 36). In particular, we found two types of genes that have never been reported in red algae: phycobilisome linker proteins and phycobilisome-degradation protein (NblA) that match to cyanobacterial homologs. Spectral characteristics. Although highly fluores-. In the filamentous N2-fixing Tolypothrix sp. 2008) and C-termini (Bienert et al. PFAM: Phycobilisome degradation protein nblA; InterPro IPR007574; KEGG: cyc:PCC7424_1012 phycobilisome degradation protein NblA; PFAM: Phycobilisome degradation. the impaired phycobilisome degradation, raising questions as to A modified Lowry protein assay kit (Pierce, USA) was used for the quantification of protein. During the last two years Prof. than the wild type and still has about half of its chlorophyll-protein complexes. phycobilisome degradation protein NblA: Graphical image of fluorescence kinetics : High: cells grown under 200 micro-mol photons m-2 s-1: Low: cells grown under 20. NblA mutants exhibit high absorbance of rod pigments under conditions that generally elicit phycobilisome degradation, implicating NblA in degradation of these pigments. Phycobilisome degradation is an ordered proteolytic process, visible by a color change of the cyanobacterial cell from blue–green to yellow–green (chlorosis). 152, 723–735. Manor, Nucleic Acid Research 33, 4128-4139 (2005). Each phycobilisome consists of a core made of allophycocyanin, from which several outwardly oriented rods made of stacked disks of phycocyanin and (if present) phycoerythrin(s) or phycoerythrocyanin. Experiments on the Quokka instrument using small angle neutron scattering (SANS) showed that cyanobacteria adjust to low light conditions dynamically by reconfiguring part of the phycobilisome, a protein complex identified with an important role in light harvesting. Figure 3C displays the expression profile of MITF proteins after Cpc treatment. After an initial period dominated by phycobilisome degradation, and by a mechanism which is still not clear, UVR induced the synthesis of new phycobiliproteins and the reassembly of phycobilisomes from newly synthesized phycobiliproteins and remaining free phycobiliproteins with a peak after 30 min of exposure. These organisms possess several types of the enzyme complexes catalyzing N2 fixation and/or H2 formation or oxidation, namely, two Mo nitrogenases, a V nitrogenase, and two hydrogenases. Hess,1,3,4,5,*,@ and M. Campbell • Zakir Hossain • Amanda M. The mode of action of NblA in this degradation process is poorly understood. Schematic diagram of a phycobilisome situated on the thylakoid membrane (A) and the energy transfer steps which include charge separation (B). Ivanov, and Norman P. NblA most likely. Phycobilisome linker proteins are phosphory-lated in Synechocystis sp. Protein separation from the integrated microfluidic chip showing the electropherogram of protein separation of PBS from 10 cells. Degradation of the cyanobacterial light harvesting complex, the phycobilisome, is a general acclimation response occurring under various stress conditions. Ben Yosef, N. However, the mechanism of how a small 7kDa protein triggers the degradation of <7MDa protein complex is unclear. Phycobilisome degradation is an ordered proteolytic process, visible by a color change of the cyanobacterial cell from blue–green to yellow–green (chlorosis). We have not We can therefore assume that the 15 kDa band corre- observed however, any proteolytic activity associated to sponds either to a NblAI oligomer or to a complex formed NblAI in our in vitro preparations, ruling out the possibility between NblAI and another small polypeptide. stabilization of phycobilisomes In agreement with published studies (e. Furthermore, degradation of the phycobilisome during nutrient-limited growth may prevent photodamage that would occur if the cells were to absorb light under conditions of metabolic arrest. Photoactivation of the orange carotenoid protein (OCP) enables its interaction with a specific, yet controversial site in the core of the light-harvesting antenna, the phycobilisome (PBS). Xiaofeng Zhang, Winfried Meining, Mark Cushman et al. Reversible inactivation of PSII reaction centers was earlier shown to occur during the initial state of photoinhibition not related to D 1 protein degradation; one of the reasons for PSII reversible inactivation is accumulation of reducing equivalents in PSII acceptor site [44, 45, 47]. A small protein, NblA, is essential for the degradation of this pigment complex under nutrient limitation. The largest differences in protein content between white and far-red light conditions reflect the changes to photosynthetic complexes, shifting from a standard phycobilisome and Chl a-based light harvesting system under white light, to modified, red-shifted phycobilisomes and Chl f-containing photosystems under far-red light conditions. Conclusion: Our data indicates that acetyl-phosphate-dependent phosphorylation of response regulator(s) overlaps with DspA-dependent signalling of the degradation of chlorophyll-protein complexes (and to a lesser extent phycobilisomes) in Synechocystis 6803. Phycobilisome degradation is an ordered proteolytic process, visible by a color change of the cyanobacterial cell from blue-green to yellow-green (chlorosis). Here we present structural and functional studies of PBCP from Oryza sativa (OsP. PCC 7942 nitrogen regulator PipX interacts in a 2-oxoglutarate 2 dependent manner with the global nitrogen transcription factor NtcA and the signal 3 transduction protein PII. Nitrogen or sulfur starvation differentially affects phycobilisome degradation and expression of the nblA gene in Synechocystis strain PCC 6803. Sherman3 and Richard D. 30 [a] The protein mass, Mw calc, was calculated for the whole sequence without the leading Met using the Compute pI/Mw tool on the ExPASy Proteomics Server. Fast D1 degradation and synthesis were confirmed by the pulse-chase and immunoblot experiments. Tikkanen, M. Cockshutt • Olga Zhaxybayeva • Hongyan Wu • Gang Li Received: 28 January 2013/Accepted: 1 March 2013 Springer Science+Business Media Dordrecht 2013 Abstract All oxygenic photoautotrophs suffer photoin-. phycobilisome in spirulina, has been suggested to exhibit radical-scavenging property [1] to reduce inflammatory responses [2,3] and oxidative stress [1,4]. The experiments were repeated four times and the phycobilisome. To regulate the effectiveness of photosynthesis and photoprotection cyanobacteria utilize a system consisting of only few components. ation prevents proteolytic degradation of deactivated D1 pro-tein in the grana stacks. 2006, 188: 5258-5265. A NblA dimer triggers the degradation of PBS com-plexes by binding to ClpC and phycobiliproteins on its N-terminus (Karradt et al. Protein knowledgebase. The phycobilisome (PBS) is a photosynthetic light‐harvesting complex in red algae, whose structural genes are separately encoded by both the nuclear and chloroplast genomes. subunit of an enzyme involved in bilin attachment to phycocyanin. nblA: Phycobilisome degradation protein NblA; Involved in phycobilisome (PBS) degradation during nutrient deprivation. " Biomolecules. One of the responses exhibited by cyanobacteria when they are limited for an essential nutrient is the rapid degradation of their light-harvesting complex, the phycobilisome. Immunolocalization of this enzyme was used as a marker of phycobilisome integrity. Phycobilisome degradation is induced by expression of the nblA gene, which encodes a protein of ∼7 kDa. PCC 6803 Sato, H. J Bacteriol 183, 2989-2994. These organisms possess several types of the enzyme complexes catalyzing N2 fixation and/or H2 formation or oxidation, namely, two Mo nitrogenases, a V nitrogenase, and two hydrogenases. Gareb and J. Increases in the cAMP concentration cause activation of glycogen degradation, increased fatty acid breakdown, stimulation of glycolysis in muscle, and stimulation of gluconeogenesis in the liver. Ammonium Induced Expression of the Red Algal Chloroplast Gene Ycf18, a Putative Homolog of the Cyanobacterial NblA Gene Involved in Nitrogen Deficiency-Induced Phycobilisome Degradation Tomohiro KAWAKAMI , Kazuaki SAKAGUCHI , Katsuaki TAKECHI , Hiroyoshi TAKANO & Susumu TAKIO. Nagarajan A, Zhou M, Nguyen AY, Liberton M, Kedia K, Shi T, Piehowski P, Shukla A, Fillmore TL, Nicora C, Smith RD, Koppenaal DW, Jacobs JM, Pakrasi HB (2019) Proteomic insights into phycobilisome degradation, a selective and tightly controlled process in the fast-growing cyanobacterium Synechococcus elongatus UTEX2973. 30 Phycobilisome degradation protein (NblA) 60 7,266. A small protein, NblA, is essential for the degradation of this pigment complex under nutrient limitation. View Amelia Nguyen's profile on LinkedIn, the world's largest professional community. 1 protein network: Involved in mRNA degradation. 001148 phycobilisome degradation protein NblA -12-15. After an initial event characterized by phycobilisome degradation following UVR exposure of Nostoc cells, UV light induced the synthesis of new phycobiliproteins and the assembly of phycobilisomes (Fig. The absorbed wavelengths, energy transfer efficiency, and time before emission depend on both the fluorophore structure and its chemical environment, as the molecule in its excited state interacts with surrounding molecules. Code APCac APCbc APCB PCC LCM LC PCac PCbc LRsm LRpc PLas PLbs LRCpc PEac PEbc LPEgc LPE LCpeS Prochlor. Interestingly, NblA, a phycobilisome degradation protein encoded by the nblA (Synpcc7942_0430), was significantly up-regulated by 1. In digestion of food, digestive enzymes may be released into the environment for extracellular digestion whereby proteolytic cleavage breaks proteins into smaller peptides and amino acids so that they may be absorbed and used. Laufman, R. Read more. Phycobilisome degradation is induced by expression of the nblA gene, which encodes a protein of ∼7 kDa. Generic labeling of proteins is achieved through fluorescent-antibody binding. This work provided biochemical characterization of HhoA, HtrA and HhoB from Synechocystis sp. Thanks to C. 8-A crystal structure of NblA from Anabaena sp. The proteolysis adaptor, NblA, binds to the N-terminus of β-phycocyanin: Implications for the mechanism of phycobilisome degradation. Protein quantification and detection methods 1) Spectroscopic procedures 2) Measurement of the total protein content by colorimetry 3) Amino acid analysis 4) Other methods, eg. Ivanov, and Norman P. PCC 6803 possess two nblA genes, nblA1 and nblA2, which are both essential for phycobilisome degradation. J Bacteriol 183, 2989-2994. Here it is shown that the new reconstitution system based on W/O emulsion is a general approach proven to work for assembly of WSCP apoproteins from Brussels sprouts, cauliflower, Japanese horseradish and Virginia. Firstly mature HhoA, HhoB and HtrA from Synechocystis sp. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. The largest differences in protein content between white and far-red light conditions reflect the changes to photosynthetic complexes, shifting from a standard phycobilisome and Chl a-based light harvesting system under white light, to modified, red-shifted phycobilisomes and Chl f-containing photosystems under far-red light conditions. Some algal walls also have an outer cuticle of protein (Hanic & Craigie 1969). J Bacteriol. Ammonium Induced Expression of the Red Algal Chloroplast Gene Ycf18, a Putative Homolog of the Cyanobacterial NblA Gene Involved in Nitrogen Deficiency-Induced Phycobilisome Degradation Tomohiro KAWAKAMI , Kazuaki SAKAGUCHI , Katsuaki TAKECHI , Hiroyoshi TAKANO & Susumu TAKIO. In this study we identified a novel mutant of Synechococcus elongatus PCC 7942 that exhibits impaired phycobilisome degradation specifically during nitrogen starvation, unlike previously described mutants, which exhibit aberrant. Previous ensemble measurements have shown that this degradation starts from the. Previous directional deep sequencing of Anabaena's transcriptome during removal of combined nitrogen from the environment identified a novel antisense RNA transcript that runs the length of and is complementary to the nblA open reading frame. in their protein structure, phycobilin content and fluorescent properties. In digestion of food, digestive enzymes may be released into the environment for extracellular digestion whereby proteolytic cleavage breaks proteins into smaller peptides and amino acids so that they may be absorbed and used. PCC 7601 the nblAI gene, encoding a NblA homologue, is located upstream of the operon coding for phycoerythrin (cpeBA). The expression of MITF protein was significantly inhibited at 540 min after Cpc (0. Accumulation of undegraded proteins in aggrosomes/inclusion bodies, or the premature degradation of (mutant) proteins is often associated with development of diseases, like cardiac and skeletal muscle myopathies. The lifespan of proteins vary gr eatly and are dependent on several factors. Abstract When deprived of an essential nutrient cyanobacteria undergo a process known as chlorosis, in which phycobilisome degradation occurs. Annotation systems. Kalim Akhtar2,6,* In metabolic engineering, the production of industrially relevant chemicals, via. In an alternative scenario, NblA may be able to associate with the large complex and thereby initiate the degradation process. Here it is shown that the new reconstitution system based on W/O emulsion is a general approach proven to work for assembly of WSCP apoproteins from Brussels sprouts, cauliflower, Japanese horseradish and Virginia. Code APCac APCbc APCB PCC LCM LC PCac PCbc LRsm LRpc PLas PLbs LRCpc PEac PEbc LPEgc LPE LCpeS Prochlor. Photosystem II protein clearance and FtsH function in the diatom Thalassiosira pseudonana Douglas A. synthesis, degradation and activity of FNR were analyzed. NblA is essential for phycobilisome degradation in Anabaena sp. Previous ensemble measurements have shown that this degradation starts from the. In: Methods in Enzymology Vol. Help pages, FAQs, UniProtKB manual, documents, news archive and Biocuration projects. PFAM: Phycobilisome degradation protein nblA; InterPro IPR007574; KEGG: cyc:PCC7424_1012 phycobilisome degradation protein NblA; PFAM: Phycobilisome degradation. Moreover, the unique genome carries a nonbleaching protein A (NblA) gene (open reading frame [ORF] 022L), which is present in all phycobilisome-containing organisms and mediates phycobilisome degradation. The experiments were repeated four times and the phycobilisome. In cyanobacteria and red alga, most of the Photosystem II associated light harvesting is performed by an enormous (3-7 MDa) membrane attached complex called the phycobilisome (PBS). To date the identity of the protease(s) involved in phycobilisome degradation has remained elusive. 30 0 0 7,266. 22LRpec Phycobilisome rod linker polypeptide, phycoerythrocyanin-associated 23NblA Phycobilisome degradation protein NblA 24NblB Phycocyanin alpha phycocyanobilin lyase related protein NblB (A) (B) Organism Var. , biosensors, photoelectric transducers). No reversible D1 phosphorylation has been observed in. Each phycobilisome consists of a core made of allophycocyanin, from which several outwardly oriented rods made of stacked disks of phycocyanin and (if present) phycoerythrin(s) or phycoerythrocyanin. phycobilisome to the cyanobacterial PSII reaction center such that centers without an intact metal cluster are not efficiently coupled with respect to energy transfer from the phycobilisome (Hwang et al. Determination of involvement of oxygen radical species in protein degradation upon high light or UVB irradiation of plant or cyanobacteria has been also carried out. The orange carotenoid protein (OCP) is a structurally and functionally modular photoactive protein involved in cyanobacterial photoprotection. Biomolecules 2019, 9 (8) , 374. Manor, Nucleic Acid Research 33, 4128-4139 (2005). We have cloned the genes encoding for the NblA. the nblA gene, an essential element for phycobilisome degradation in Synechococcus 2973, as a target for dele-tion [32]. Third, in the prokaryotic cyanobacteria and eukaryotic red algae, light harvesting is carried out primarilyby agroup of. dnaN protein network: https://string-db. Journal of molecular biology, Vol. Comparison of psbO and psbH deletion mutants of Synechocystis PCC 6803 indicates that degradation of D1 protein is regulated by the QB site and dependent on protein synthesis. 313-318, Academic Press; and Gantt et al. We respectively link a Gal4 binding domain with CikA or SasA to find the suitable binding protein that can characterize the oscillation. , a nblA gene (nblAI) lies upstream of the cpeBA operon that encodes phycoerythrin apoproteins. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. Targeted protein degraders are redefining how small molecules look and act The new modality promises to open up the human proteome to drug developers, but first they will need to work out the. Sequence clusters. NblA most likely. Binding assays showed interaction between Slr1098. , Hernández-Prieto, M. The second plasmid is our reporter plasmid, which has fluorescent protein promoted by mutant Gal1p and luciferase promoted by Gal2p. Nguyen et al. The Bradford Method is the most common procedure used in research laboratories to determine protein amounts The assay is based on the absorbance shift that occurs from 465 to 595 nm when the dye binds to protein in an acidic environment. protein degradation mechanisms The regulated degradation of proteins is an important mechanism for the survival of cells. We combine protein signatures from a number of member databases into a single searchable resource, capitalising on their individual strengths to produce a powerful integrated database and diagnostic tool. At high light, PBPs potentially induce photodamage by overexcitation of the reaction centers.